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Phytophthora infestans (Mont.) de Bary. I. Host-pathogen specificity and resistance components

TitlePhytophthora infestans (Mont.) de Bary. I. Host-pathogen specificity and resistance components
Publication TypeJournal Article
2006
AuthorsLozoya-Saldana, H, Guzman-Galindo, L, Fernandez-Pavia, SP, Grünwald, NJ, McElhinny, E
JournalAgrociencia
Volume40
Pagination205-217
Date PublishedMar-Apr
1405-3195
ISI:000236252100006
central mexico, clonal lineages, compatibility, field-resistance, genetic differentiation, genetic resistance, host-pathogen specificity, isozymes, late blight, mating types, metalaxyl, metalaxyl resistance, population-structure, solanum, toluca valley

The molecular identity of Phytophthora infestans strains infecting potatoes in the Toluca Valley has been documented. Nevertheless, the relationship of the P infestans genotypes to the host's genetic resistance is unclear. In order to identify potential host-pathogen specificity, isolates of the oomycete obtained from five potato varieties with differing levels of disease resistance were compared regarding mating type (MT), isozyme genotype for glucose-6-phosphate isomerase (Gpi) and peptidase (Pep), and sensitivity to metalaxyl. Also, host resistance components and specificity on the host-pathogen interaction were assessed twice in detached leaves in the laboratory and once in attached leaves in the greenhouse for three of the varieties. No mating type predominated relative to host genetic resistance (frequency of A1:A2; Alpha, susceptible: 1:1.6; Rosita, resistant: 1:0.75; Nortena, resistant: 1:0.9; Monserrat, resistant: 1:2; Michoacan, resist-ant: 1:0.20). Eighteen MT-isozyme combinations were identified from 97 isolates. All of them have been previously reported. The most common multilocus genotypes (MT, Gpi, and Pep) were A1, 86/100, 100/100 (37% of the whole population) and A2, 86/100, 100/100 (14%). Both genotypes represented 66% of the P. infestans population obtained from cvs. Michoacan and Monserrat. The level of metalaxyl sensitivity was lower relative to the levels reported in previous studies. A relationship between frequency of pathogen genotypes and level of host genetic resistance was not observed. The isolate from Rosita was the most aggressive. It resulted in the largest lesion area in the laboratory and in the greenhouse,, and showed the highest sporulation in the greenhouse. The isolate from Alpha, which showed the highest sporulation in the laboratory, was second in sporulation under greenhouse conditions. Aggressiveness was higher in isolates obtained from resistant varieties and only limited host-pathogen specificity was observed in cvs. Alpha and Rosita. Suppression of lesion area and sporulation were the host resistance components identified only for the variety Nortena.

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