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Microbe-ID: an open source toolbox for microbial genotyping and species identification

TitleMicrobe-ID: an open source toolbox for microbial genotyping and species identification
Publication TypeJournal Article
2016
AuthorsTabima, JF, Everhart, SE, Larsen, MM, Weisberg, AJ, Kamvar, ZN, Tancos, MA, Smart, CD, Chang, JH, Grünwald, NJ
JournalPeerJ
Volume4
Paginatione2279
ISSN2167-8359
Genotyping, identification, Molecular diagnostics, pathogen, phytophthora, taxonomy

Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus \textit{Phytophthora} (phytophthora-id.org). In \textit{Phytophthora}-ID, the Sequence-ID application allows identification based on ITS or \textit{cox} spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species \textit{P. infestans} and \textit{P. ramorum}. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID.

https://doi.org/10.7717/peerj.2279
10.7717/peerj.2279
Citation Key10.7717/peerj.2279