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Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum

TitleIdentification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum
Publication TypeJournal Article
2011
AuthorsVercauteren, A, Larsen, M, Goss, EM, Grünwald, NJ, Maes, M, Heungens, K
JournalMycologia
Volume103
Pagination1245-1249
Date PublishedNov-Dec
0027-5514
ISI:000297387900009
california, clonal lineages, genetic diversity, microsatellite markers, migration, north-america, nursery, oak death pathogen, oomycetes, sudden oak death, universal labeling

Phytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the limited intralineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphic microsatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified and as such can help better characterize the North American lineages of P. ramorum.

10.3852/10-420
Citation Key119

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