You are here

Analysis of genotypic diversity data for populations of microorganisms

TitleAnalysis of genotypic diversity data for populations of microorganisms
Publication TypeJournal Article
2003
AuthorsGrünwald, NJ, Goodwin, SB, Milgroom, MG, Fry, WE
JournalPhytopathology
Volume93
Pagination738-746
Date PublishedJun
0031-949X
ISI:000183071900013
chestnut blight fungus, cryphonectria-parasitica, dynamics, epidemiology, evenness, genetic-structure, microbial ecology, mycosphaerella-graminicola, pathogen populations, phytophthora-infestans, population genetics, setosphaeria-turcica populations, sexual reproduction

Estimation of genotypic diversity is an important component of the analysis of the genetic structure of plant pathogen and microbial populations. Estimates of genotypic diversity are a function of both the number of genotypes observed in a sample (genotype richness) and the evenness of distribution of genotypes within the sample. Currently used measures of genotypic diversity have inherent problems that could lead to incorrect conclusions, particularly when diversity is low or sample sizes differ. The number of genotypes observed in a sample depends on the technique used to assay for genetic variation; each technique will affect the maximum number of genotypes that can be detected. We developed an approach to analysis of genotypic diversity in plant pathology that makes specific reference to the techniques used for identifying genotypes. Preferably, populations that are being compared should be very similar in sample size. In this case, the number of genotypes observed can be used directly for comparing richness. In most cases, sample sizes differ and use of the rarefaction method to calculate richness is more appropriate. In all cases, scaling either Stoddart and Taylor's G or Shannon and Wiener's H' by sample size should be avoided. Under those circumstances where it might be important to distinguish whether richness or evenness contribute more to diversity, a bootstrapping approach, where confidence intervals are calculated for indices of diversity and evenness, is recommended.

Citation Key189